Hotspot

secG

Genome · 104434-104634 · 201 bp

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Pi 0.244

MarkerSeek score 72.50

Primer available yes

Label secG

Feature Metadata

label_name: secG
feature_type: gene
region: Genome
length_bp: 201
variable_sites: 49
pi: 0.244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
secG_p1	TTTGAACCACTGACCTTCGG	AGTTTGGGGTAGAAACGGTT	50.0	45.0	58.0	57.0	1261 bp	0.5	51.4
secG_p2	ATCCAATTTTGCCCGTCGTA	AGTTTGGGGTAGAAACGGTT	45.0	45.0	57.9	57.0	1030 bp	0.5	50.8
secG_p3	AGAATAGCGGGGAGTGGATT	AGTTTGGGGTAGAAACGGTT	50.0	45.0	58.2	57.0	1279 bp	0.5	50.2
secG_p4	AGCTACCAGACTGCTCTACC	AGTTTGGGGTAGAAACGGTT	55.0	45.0	58.2	57.0	1226 bp	0.5	49.9
secG_p5	TTTGAACCACTGACCTTCGG	AGTTTGGGGTAGAAACGGTTT	50.0	42.9	58.0	57.7	1261 bp	0.5	49.3