Hotspot

ntcA

Genome · 152833-153432 · 600 bp

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Pi 0.244

MarkerSeek score 80.40

Primer available yes

Label ntcA

Feature Metadata

label_name: ntcA
feature_type: gene
region: Genome
length_bp: 600
variable_sites: 144
pi: 0.244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ntcA_p1	CTTTGCCCTACGCTTACACT	ATTATACGCGGGTTCGAGTC	50.0	50.0	57.9	57.6	2003-2013 bp	1.0	85.1
ntcA_p2	CTTTGCCCTACGCTTACACT	AACCAGGTCAGGAGACTCTT	50.0	50.0	57.9	57.6	1494 bp	0.5	56.0
ntcA_p3	CTTTGCCCTACGCTTACACT	ACCAGGTCAGGAGACTCTTT	50.0	50.0	57.9	57.6	1493 bp	0.5	56.0
ntcA_p4	CTTTGCCCTACGCTTACACT	TTGTGGCTCCATTATACGCG	50.0	50.0	57.9	58.4	2013 bp	0.5	55.9
ntcA_p5	TGCTTCTGCTACAGTTGGTC	ACCAGGTCAGGAGACTCTTT	50.0	50.0	57.8	57.6	1155 bp	0.5	55.4