Hotspot

moeB

Genome · 148448-149524 · 1077 bp

Back to Bostrychia

Pi 0.222

MarkerSeek score 81.00

Primer available yes

Label moeB

Feature Metadata

label_name: moeB
feature_type: gene
region: Genome
length_bp: 1077
variable_sites: 234
pi: 0.222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB_p1	ATGCATATCCTGGAGACACA	GAAGGACTTGAACCCTCGAC	45.0	55.0	56.0	57.9	1390 bp	0.5	43.3
moeB_p2	TGCATATCCTGGAGACACAT	GAAGGACTTGAACCCTCGAC	45.0	55.0	56.0	57.9	1389 bp	0.5	43.3
moeB_p3	ATGCATATCCTGGAGACACA	CATGTTGACCTAAAGCCGGA	45.0	50.0	56.0	57.9	1680 bp	0.5	43.1
moeB_p4	TGCATATCCTGGAGACACAT	CATGTTGACCTAAAGCCGGA	45.0	50.0	56.0	57.9	1679 bp	0.5	43.1
moeB_p5	TGCATATCCTGGAGACACAT	ATGTTGACCTAAAGCCGGAC	45.0	50.0	56.0	57.9	1678 bp	0.5	43.1