Hotspot

dnaB

Genome · 145984-147852 · 1869 bp

Back to Bostrychia

Pi 0.227

MarkerSeek score 80.10

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1869
variable_sites: 415
pi: 0.227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	TCTACCAGCTGAGCTATCCC	ATGTGTCTCCAGGATATGCA	55.0	45.0	58.0	56.0	2366 bp	0.5	43.9
dnaB_p2	TCTACCAGCTGAGCTATCCC	TGTGTCTCCAGGATATGCAT	55.0	45.0	58.0	56.0	2365 bp	0.5	43.9
dnaB_p3	TTCAGTCCACTGCTCTACCA	ATGTGTCTCCAGGATATGCA	50.0	45.0	58.0	56.0	2379 bp	0.5	43.9
dnaB_p4	TTCAGTCCACTGCTCTACCA	TGTGTCTCCAGGATATGCAT	50.0	45.0	58.0	56.0	2378 bp	0.5	43.9
dnaB_p5	TTTGAACTGGTGACACGAGG	TGTGTCTCCAGGATATGCAT	50.0	45.0	58.1	56.0	2401 bp	0.5	43.6