Hotspot

carA

Genome · 12549-13724 · 1176 bp

Back to Bostrychia

Pi 0.191

MarkerSeek score 79.70

Primer available yes

Label carA

Feature Metadata

label_name: carA
feature_type: gene
region: Genome
length_bp: 1176
variable_sites: 223
pi: 0.191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
carA_p1	GAAATGGGGAGGAGGCAAAA	AGCACCTGAAGGGCATTTAC	50.0	50.0	58.1	58.2	1892 bp	0.5	58.2
carA_p2	CTGCTTGTCATTCTGGTGGT	AGCACCTGAAGGGCATTTAC	50.0	50.0	58.1	58.2	1606 bp	0.5	57.9
carA_p3	GAAATGGGGAGGAGGCAAAA	CACCTGAAGGGCATTTACCA	50.0	50.0	58.1	57.8	1890 bp	0.5	57.8
carA_p4	CTGCTTGTCATTCTGGTGGT	CACCTGAAGGGCATTTACCA	50.0	50.0	58.1	57.8	1604 bp	0.5	57.5
carA_p5	TGTTGCTGAGAAATGGGGAG	AGCACCTGAAGGGCATTTAC	50.0	50.0	57.8	58.2	1901 bp	0.5	57.1