Hotspot

orf65-orf40

Genome · 45407-45979 · 573 bp

Back to Bostrychia

Pi 0.373

MarkerSeek score 80.40

Primer available yes

Label orf65-orf40

Feature Metadata

label_name: orf65-orf40
feature_type: igs
region: Genome
length_bp: 573
variable_sites: 209
pi: 0.373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf65-orf40_p1	AGCCGGACAAATAGCTTCAG	AGCCTCACCTTGACCTAGTT	50.0	50.0	58.0	58.0	1684-2359 bp	1.0	88.0
orf65-orf40_p2	TGGTTGTAAAGCCGGACAAA	AGCCTCACCTTGACCTAGTT	45.0	50.0	57.9	58.0	1693-2368 bp	1.0	87.8
orf65-orf40_p3	AGCCGGACAAATAGCTTCAG	ACGACCTACCCCACCAATTA	50.0	50.0	58.0	58.0	1465 bp	0.5	59.4
orf65-orf40_p4	AGCCGGACAAATAGCTTCAG	CGATAACCACCAGGAGAAGC	50.0	55.0	58.0	58.1	1566 bp	0.5	59.3
orf65-orf40_p5	AGCCGGACAAATAGCTTCAG	GCGATAACCACCAGGAGAAG	50.0	55.0	58.0	58.1	1567 bp	0.5	59.3