Hotspot

ConsOrf5

Genome · 63404-64468 · 1065 bp

Back to Bostrychia

Pi 0.291

MarkerSeek score 82.00

Primer available yes

Label ConsOrf5

Feature Metadata

label_name: ConsOrf5
feature_type: gene
region: Genome
length_bp: 1065
variable_sites: 299
pi: 0.291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ConsOrf5_p1	AACCAGCGTTGTCCTACAAG	TTAAAAGCATCTGTGGCCGA	50.0	45.0	58.1	57.8	1856 bp	0.5	57.5
ConsOrf5_p2	GATTCCCACTACCCGCTTTT	TTAAAAGCATCTGTGGCCGA	50.0	45.0	57.9	57.8	1908 bp	0.5	57.5
ConsOrf5_p3	GAACCAGCGTTGTCCTACAA	TTAAAAGCATCTGTGGCCGA	50.0	45.0	58.1	57.8	1857 bp	0.5	57.4
ConsOrf5_p4	AACCAGCGTTGTCCTACAAG	TAAAAGCATCTGTGGCCGAG	50.0	50.0	58.1	58.3	1855 bp	0.5	56.9
ConsOrf5_p5	GATTCCCACTACCCGCTTTT	TAAAAGCATCTGTGGCCGAG	50.0	50.0	57.9	58.3	1907 bp	0.5	56.9