Hotspot

ndhD-psaC

SSC · 118068-118266 · 199 bp

Back to Boronia

Pi 0.0535

MarkerSeek score 76.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 199
variable_sites: 10
pi: 0.0535
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TAAAGAACCCGCGGCTATTG	AAACAACTCGAAGCATGGGT	50.0	45.0	58.3	58.0	252-264 bp	1.0	83.9
ndhD-psaC_p2	TAAAGAACCCGCGGCTATTG	GGCATGAAACAACTCGAAGC	50.0	50.0	58.3	58.0	258-270 bp	1.0	83.7
ndhD-psaC_p3	AAAATTAAAGAACCCGCGGC	AAACAACTCGAAGCATGGGT	45.0	45.0	57.6	58.0	257-269 bp	1.0	83.7
ndhD-psaC_p4	AAAATTAAAGAACCCGCGGC	GGCATGAAACAACTCGAAGC	45.0	50.0	57.6	58.0	263-275 bp	1.0	83.4
ndhD-psaC_p5	TTAAAGAACCCGCGGCTATT	AAACAACTCGAAGCATGGGT	45.0	45.0	57.6	58.0	253-265 bp	1.0	83.4