Hotspot

trnN-GUU-rpl32

SSC · 109492-113977 · 4486 bp

Back to Boronia

Pi 0.0322

MarkerSeek score 63.30

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: SSC
length_bp: 4486
variable_sites: 136
pi: 0.0322
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	TGTTGCGGTCGGAAAAAGTA	AGGGGTTAGTTTCGAGAAAAGA	45.0	40.9	58.0	57.0	865 bp	0.5	35.9
trnN-GUU-rpl32_p2	GTTTTGTTGCGGTCGGAAAA	AGGGGTTAGTTTCGAGAAAAGA	45.0	40.9	58.1	57.0	869 bp	0.5	35.5
trnN-GUU-rpl32_p3	TTTGTTGCGGTCGGAAAAAG	AGGGGTTAGTTTCGAGAAAAGA	45.0	40.9	57.8	57.0	867 bp	0.5	34.7
trnN-GUU-rpl32_p4	CCACATACGGCGAAGATGTT	AGGGGTTAGTTTCGAGAAAAGA	50.0	40.9	58.3	57.0	886 bp	0.5	33.5
trnN-GUU-rpl32_p5	TGTTGCGGTCGGAAAAAGTA	AAGGGGTTAGTTTCGAGAAAAGA	45.0	39.1	58.0	57.7	866 bp	0.5	33.2