Hotspot

rpl14-rpl16

LSC · 81344-81468 · 125 bp

Back to Boronia

Pi 0.107

MarkerSeek score 75.20

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 125
variable_sites: 11
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CACCACTATTGTCCGCTACA	TGCCTATAAGAACTCAATTCA	50.0	33.3	57.3	52.3	184-206 bp	1.0	48.5
rpl14-rpl16_p2	GCACCACTATTGTCCGCTAC	TGCCTATAAGAACTCAATTCA	55.0	33.3	58.7	52.3	185-207 bp	1.0	48.5
rpl14-rpl16_p3	ACACATCAATTCTCGGGCAC	TGCCTATAAGAACTCAATTCA	50.0	33.3	58.5	52.3	201-223 bp	1.0	48.2
rpl14-rpl16_p4	ACACATCAATTCTCGGGCAC	TGCCTATAAGAACTCAATTCAT	50.0	31.8	58.5	52.9	201-223 bp	1.0	48.2
rpl14-rpl16_p5	ACACATCAATTCTCGGGCAC	ATGCCTATAAGAACTCAATTCA	50.0	31.8	58.5	52.9	202-224 bp	1.0	48.1