Hotspot

trnW-CCA-trnP-UGG

LSC · 66741-66921 · 181 bp

Back to Boronia

Pi 0.0468
MarkerSeek score 74.70
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
181
variable_sites
8
pi
0.0468
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 238-247 bp 1.0 84.8
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 240-249 bp 1.0 84.7
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCACGTTCT TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 241-250 bp 1.0 84.7
trnW-CCA-trnP-UGG_p4 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 251-260 bp 1.0 84.5
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 252-261 bp 1.0 84.5