Hotspot

petG-trnW-CCA

LSC · 66524-66667 · 144 bp

Back to Boronia

Pi 0.0563

MarkerSeek score 74.60

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 8
pi: 0.0563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	223-226 bp	1.0	82.4
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	245-248 bp	1.0	82.2
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	243-246 bp	1.0	79.6
petG-trnW-CCA_p4	GTGGTGATCAGTTGGACCTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.4	58.0	239-242 bp	1.0	79.0
petG-trnW-CCA_p5	CAGACGTGGTGATCAGTTGG	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.6	58.0	244-247 bp	1.0	78.9