Hotspot

trnfM-CAU-rps14

LSC · 37469-37631 · 163 bp

Back to Boronia

Pi 0.0625
MarkerSeek score 75.50
Primer available yes
Label trnfM-CAU-rps14

Feature Metadata

label_name
trnfM-CAU-rps14
feature_type
igs
region
LSC
length_bp
163
variable_sites
10
pi
0.0625
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-rps14_p1 GCTACCAAACTGCTCTACCC CGTTGTTTTTCGACCGGAAG 55.0 50.0 58.0 58.0 304-307 bp 1.0 83.1
trnfM-CAU-rps14_p2 AAGGTTATGAGCCTTGCGAG CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.0 58.0 323-326 bp 1.0 82.9
trnfM-CAU-rps14_p3 GAACCCGTGACTTCAAGGTT CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.0 58.0 337-340 bp 1.0 82.7
trnfM-CAU-rps14_p4 TTGAACCCGTGACTTCAAGG CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.0 58.0 339-342 bp 1.0 82.6
trnfM-CAU-rps14_p5 GGGATTTGAACCCGTGACTT CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.1 58.0 344-347 bp 1.0 82.1