Hotspot

trnT-GGU-psbD

LSC · 32114-33307 · 1194 bp

Back to Boronia

Pi 0.0441

MarkerSeek score 77.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1194
variable_sites: 52
pi: 0.0441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AAAAACGAATCGGTCCCTCC	55.0	50.0	58.1	58.2	1341-1342 bp	1.0	82.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	AAAAACGAATCGGTCCCTCC	50.0	50.0	58.1	58.2	1344-1345 bp	1.0	81.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1357-1358 bp	1.0	81.4
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAATCGGTCCCT	55.0	45.0	58.1	57.7	1343-1344 bp	1.0	81.4
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1360-1361 bp	1.0	81.2