Hotspot

psbK-psbI

LSC · 6957-7351 · 395 bp

Back to Boronia

Pi 0.0522

MarkerSeek score 74.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 20
pi: 0.0522
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	AGACAGGAATCCGAAGATGA	50.0	45.0	57.9	55.7	490-500 bp	1.0	66.0
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	GACAGGAATCCGAAGATGAAGA	50.0	45.5	57.9	57.6	489-499 bp	1.0	65.6
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	ACAGGAATCCGAAGATGAAGAG	50.0	45.5	57.9	57.6	488-498 bp	1.0	65.6
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	AGACAGGAATCCGAAGATGAAG	50.0	45.5	57.9	57.6	490-500 bp	1.0	65.5
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	ACAGGAATCCGAAGATGAAGA	50.0	42.9	57.9	56.5	488-498 bp	1.0	64.3