Hotspot

rps16-trnQ-UUG

LSC · 5875-6340 · 466 bp

Back to Boronia

Pi 0.0649
MarkerSeek score 74.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
466
variable_sites
30
pi
0.0649
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GGTTCGAATCCTTCCGTCC 50.0 57.9 58.0 57.9 555-558 bp 1.0 76.2
rps16-trnQ-UUG_p2 CAGCGGATCATGTCCTTCAA GTTCGAATCCTTCCGTCCC 50.0 57.9 58.0 57.9 554-557 bp 1.0 76.2
rps16-trnQ-UUG_p3 GCGGATCATGTCCTTCAAGT GGTTCGAATCCTTCCGTCC 50.0 57.9 58.0 57.9 553-556 bp 1.0 76.2
rps16-trnQ-UUG_p4 GCGGATCATGTCCTTCAAGT GTTCGAATCCTTCCGTCCC 50.0 57.9 58.0 57.9 552-555 bp 1.0 76.2
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GTTCGAATCCTTCCGTCCC 45.0 57.9 58.1 57.9 536-539 bp 1.0 75.5