Hotspot

trnK-UUU-rps16

LSC · 4154-4717 · 564 bp

Back to Boronia

Pi 0.0581
MarkerSeek score 78.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
564
variable_sites
32
pi
0.0581
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT ACAGAACTTAGTCTTAATCAAACGA 50.0 32.0 58.2 56.5 634-726 bp 1.0 45.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT ACAGAACTTAGTCTTAATCAAACGA 50.0 32.0 58.2 56.5 633-725 bp 1.0 45.3
trnK-UUU-rps16_p3 CTCCCGAAGATTTGGACGAA ACAGAACTTAGTCTTAATCAAACGA 50.0 32.0 57.6 56.5 698-790 bp 1.0 44.8
trnK-UUU-rps16_p4 TTCTCCCGAAGATTTGGACG ACAGAACTTAGTCTTAATCAAACGA 50.0 32.0 57.6 56.5 700-792 bp 1.0 44.8
trnK-UUU-rps16_p5 TCTCCCGAAGATTTGGACGA ACAGAACTTAGTCTTAATCAAACGA 50.0 32.0 58.4 56.5 699-791 bp 1.0 44.8