Hotspot

trnH-GUG-psbA

LSC · 72-215 · 144 bp

Back to Boronia

Pi 0.107

MarkerSeek score 75.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 15
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	604-861 bp	1.0	81.6
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	CAGTGCTATGCATGGTTCCT	50.0	50.0	57.9	58.0	603-860 bp	1.0	81.6
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	TCAGTGCTATGCATGGTTCC	50.0	50.0	58.2	58.0	605-862 bp	1.0	81.4
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	CAGTGCTATGCATGGTTCCT	50.0	50.0	58.2	58.0	604-861 bp	1.0	81.4
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	ATGCACCCATTCCACATGTT	50.0	45.0	57.9	58.1	656-913 bp	1.0	81.3