Hotspot

rpl16

LSC · 84468-86124 · 1657 bp

Back to Borassus

Pi 0.00123
MarkerSeek score 60.70
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1657
variable_sites
2
pi
0.00123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AAAAAGAAACCTGCGGTTGT ACAAACTCTTTCATTCTTTTTCTGT 40.0 28.0 56.7 56.1 1814-1818 bp 1.0 41.3
rpl16_p2 AAAAGAAACCTGCGGTTGTT ACAAACTCTTTCATTCTTTTTCTGT 40.0 28.0 56.7 56.1 1813-1817 bp 1.0 41.3
rpl16_p3 AAAGAAACCTGCGGTTGTTT ACAAACTCTTTCATTCTTTTTCTGT 40.0 28.0 56.7 56.1 1812-1816 bp 1.0 41.3
rpl16_p4 AAGAAACCTGCGGTTGTTTT ACAAACTCTTTCATTCTTTTTCTGT 40.0 28.0 56.7 56.1 1811-1815 bp 1.0 41.3
rpl16_p5 AGAAACCTGCGGTTGTTTTT ACAAACTCTTTCATTCTTTTTCTGT 40.0 28.0 56.7 56.1 1810-1814 bp 1.0 41.3