Hotspot

atpF

LSC · 12536-13900 · 1365 bp

Back to Borassus

Pi 0.0022

MarkerSeek score 60.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1365
variable_sites: 3
pi: 0.0022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCCT	45.0	37.5	57.4	57.5	1521 bp	1.0	40.2
atpF_p2	GGCTCGAAGGGTTACCATTA	AGAACTCTGTTCTTTGTTAGTCCT	50.0	37.5	57.0	57.5	1509 bp	1.0	40.2
atpF_p3	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCC	45.0	39.1	57.4	56.2	1521 bp	1.0	40.2
atpF_p4	ACTAATTTCGTCGGCTCGAA	GAACTCTGTTCTTTGTTAGTCCT	45.0	39.1	57.4	56.2	1520 bp	1.0	40.2
atpF_p5	ACTAATTTCGTCGGCTCGAA	ACTCTGTTCTTTGTTAGTCCT	45.0	38.1	57.4	54.1	1518 bp	1.0	40.2