Hotspot

petN-psbM

LSC · 30295-31053 · 759 bp

Back to Borassus

Pi 0.00396

MarkerSeek score 61.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 3
pi: 0.00396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTCTCACTTGGGCTGCTTTA	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.8	58.3	915-917 bp	1.0	76.9
petN-psbM_p2	GTCTCACTTGGGCTGCTTTA	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.8	57.6	916-918 bp	1.0	75.9
petN-psbM_p3	ACTTGGGCTGCTTTAATGGT	ATTGCTACTGCGCTGTTCAT	45.0	45.0	57.7	58.3	910-912 bp	1.0	75.9
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.6	58.3	860-862 bp	1.0	75.6
petN-psbM_p5	GTCTCACTTGGGCTGCTTTA	TTGCTACTGCGCTGTTCATT	50.0	45.0	57.8	58.5	914-916 bp	1.0	75.1