Hotspot

rpoB-trnC-GCA

LSC · 27808-29027 · 1220 bp

Back to Borassus

Pi 0.00164

MarkerSeek score 59.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1220
variable_sites: 2
pi: 0.00164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1368 bp	1.0	74.5
rpoB-trnC-GCA_p2	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	57.5	1288 bp	1.0	72.6
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1405 bp	1.0	72.6
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	57.5	1289 bp	1.0	72.4
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTG	GACACCCGGATTTGAACTGG	50.0	55.0	58.2	58.8	1391 bp	1.0	72.0