Hotspot

ycf1

SSC · 127763-133195 · 5433 bp

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Pi 0.00607

MarkerSeek score 43.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5433
variable_sites: 33
pi: 0.00607
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAACCAATGAAATACCTACGGT	ATGGAATGCCCCCTTGAATC	39.1	50.0	57.8	57.9	261 bp	1.0	55.4
ycf1_p2	TCAACCAATGAAATACCTACGGT	GATGGAATGCCCCCTTGAAT	39.1	50.0	57.8	57.9	262 bp	1.0	55.4
ycf1_p3	TCAACCAATGAAATACCTACGGT	GGAATGCCCCCTTGAATCAA	39.1	50.0	57.8	58.1	259 bp	1.0	55.1
ycf1_p4	CAACCAATGAAATACCTACGGT	ATGGAATGCCCCCTTGAATC	40.9	50.0	56.4	57.9	260 bp	1.0	52.2
ycf1_p5	CAACCAATGAAATACCTACGGT	GATGGAATGCCCCCTTGAAT	40.9	50.0	56.4	57.9	261 bp	1.0	52.2