Hotspot

rpl22

LSC · 86305-86691 · 387 bp

Back to Boophone

Pi 0.0155

MarkerSeek score 50.90

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 387
variable_sites: 6
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCAAGTCTGAAACCAAGTGGA	GATACTTACATTGGCGGGGG	47.6	55.0	58.1	58.1	500 bp	1.0	71.5
rpl22_p2	GTGCGAACCAAAAGGAATGA	GATACTTACATTGGCGGGGG	45.0	55.0	56.9	58.1	535 bp	1.0	71.4
rpl22_p3	CCAAGTCTGAAACCAAGTGGA	TTACATTGGCGGGGGATAAC	47.6	50.0	58.1	57.6	495 bp	1.0	69.6
rpl22_p4	GTGCGAACCAAAAGGAATGA	TTACATTGGCGGGGGATAAC	45.0	50.0	56.9	57.6	530 bp	1.0	69.5
rpl22_p5	ACCAAGTCTGAAACCAAGTGG	GATACTTACATTGGCGGGGG	47.6	55.0	58.3	58.1	501 bp	1.0	69.3