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psbA

LSC · 97-1158 · 1062 bp

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Pi 0

MarkerSeek score 28.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GGTATGGCTCCTTCAACGAT	GTGCTTGGGAGTCCTTGAAA	50.0	50.0	57.4	58.0	1154 bp	1.0	74.9
psbA_p2	TGGCTCCTTCAACGATTCTT	GTGCTTGGGAGTCCTTGAAA	45.0	50.0	57.1	58.0	1150 bp	1.0	73.0
psbA_p3	GGTATGGCTCCTTCAACGAT	TAGTACGTGTGCTTGGGAGT	50.0	50.0	57.4	58.4	1162 bp	1.0	72.0
psbA_p4	GGTATGGCTCCTTCAACGAT	GTTAGTACGTGTGCTTGGGA	50.0	50.0	57.4	57.6	1164 bp	1.0	71.5
psbA_p5	GGTATGGCTCCTTCAACGAT	AGTTAGTACGTGTGCTTGGG	50.0	50.0	57.4	57.5	1165 bp	1.0	71.4