Hotspot

ndhB-trnL-CAA

IRa · 147754-148330 · 577 bp

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Pi 0.00181

MarkerSeek score 63.20

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 577
variable_sites: 1
pi: 0.00181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TTCTTGCAATTGCTCAGGGT	GTCTCGCGTGTCTACCATTT	45.0	50.0	58.0	58.0	618-641 bp	1.0	83.0
ndhB-trnL-CAA_p2	TTCTTGCAATTGCTCAGGGT	CTCGCGTGTCTACCATTTCA	45.0	50.0	58.0	58.0	616-639 bp	1.0	83.0
ndhB-trnL-CAA_p3	TTCTTGCAATTGCTCAGGGT	GCGTGTCTACCATTTCACCA	45.0	50.0	58.0	58.2	613-636 bp	1.0	81.5
ndhB-trnL-CAA_p4	TTCTTGCAATTGCTCAGGGT	TCTCGCGTGTCTACCATTTC	45.0	50.0	58.0	57.7	617-640 bp	1.0	80.9
ndhB-trnL-CAA_p5	TTCTTGCAATTGCTCAGGGT	TCGCGTGTCTACCATTTCAC	45.0	50.0	58.0	58.3	615-638 bp	1.0	80.7