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rps15-ycf1

SSC · 127380-127762 · 383 bp

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Pi 0.0345

MarkerSeek score 63.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 383
variable_sites: 13
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCTTGTCGAATAGCTGAGATGA	ACCAATAACGGCGGTCATTT	40.9	45.0	56.7	58.2	484-487 bp	1.0	55.7
rps15-ycf1_p2	TCTTGTCGAATAGCTGAGATGA	GATACCAATAACGGCGGTCA	40.9	50.0	56.7	57.8	487-490 bp	1.0	55.2
rps15-ycf1_p3	TCTTGTCGAATAGCTGAGATGA	TGATACCAATAACGGCGGTC	40.9	50.0	56.7	57.8	488-491 bp	1.0	55.2
rps15-ycf1_p4	TCTTGTCGAATAGCTGAGATGA	ACGGCGGTCATTTCAGTATG	40.9	50.0	56.7	58.3	477-480 bp	1.0	54.4
rps15-ycf1_p5	TCTTGTCGAATAGCTGAGATGA	CGGCGGTCATTTCAGTATGT	40.9	50.0	56.7	58.3	476-479 bp	1.0	54.4