Hotspot

psaC-ndhE

SSC · 120868-121542 · 675 bp

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Pi 0.047

MarkerSeek score 76.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 675
variable_sites: 26
pi: 0.047
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	AGCTATTGTTTCGTCGATCT	50.0	40.0	58.1	54.6	740-850 bp	1.0	58.5
psaC-ndhE_p2	CCAATACATCTGTGGGGCAA	AGCTATTGTTTCGTCGATCT	50.0	40.0	57.9	54.6	717-827 bp	1.0	58.5
psaC-ndhE_p3	AATACATCTGTGGGGCAAGC	AGCTATTGTTTCGTCGATCT	50.0	40.0	58.2	54.6	715-825 bp	1.0	57.8
psaC-ndhE_p4	AGCTTTACATCCGTCCCAAG	AGCTATTGTTTCGTCGATCT	50.0	40.0	57.6	54.6	746-856 bp	1.0	56.3
psaC-ndhE_p5	TTCCAATACATCTGTGGGGC	AGCTATTGTTTCGTCGATCT	50.0	40.0	57.6	54.6	719-829 bp	1.0	56.1