Hotspot

rpl32-trnL-UAG

SSC · 116838-117631 · 794 bp

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Pi 0.00824
MarkerSeek score 67.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
794
variable_sites
6
pi
0.00824
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCAAAGTCAGTTTCTACCGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.2 57.9 841-906 bp 1.0 73.7
rpl32-trnL-UAG_p2 GCAAAGTCAGTTTCTACCGGA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.2 57.9 840-905 bp 1.0 73.7
rpl32-trnL-UAG_p3 GCAAAGTCAGTTTCTACCGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 56.7 57.9 840-905 bp 1.0 73.5
rpl32-trnL-UAG_p4 AGCAAAGTCAGTTTCTACCGG AGCAGCGTGTCTACCAATTT 47.6 45.0 58.2 57.8 834-899 bp 1.0 73.2
rpl32-trnL-UAG_p5 GCAAAGTCAGTTTCTACCGGA AGCAGCGTGTCTACCAATTT 47.6 45.0 58.2 57.8 833-898 bp 1.0 73.2