Hotspot

ndhF-rpl32

SSC · 115696-116663 · 968 bp

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Pi 0.00892
MarkerSeek score 64.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
968
variable_sites
7
pi
0.00892
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCTGTCGGAATAAGCA AGAAAAAGCTTTTATGGCAGC 45.0 38.1 57.8 55.6 984-1166 bp 1.0 56.3
ndhF-rpl32_p2 TGTTGCTGTCGGAATAAGCA AAGAAAAAGCTTTTATGGCAGC 45.0 36.4 57.8 56.4 985-1167 bp 1.0 54.2
ndhF-rpl32_p3 TGTTGCTGTCGGAATAAGCA TGGCAGCTAAATATCCCTTT 45.0 40.0 57.8 54.4 970-1152 bp 1.0 53.9
ndhF-rpl32_p4 TGTTGCTGTCGGAATAAGCA TGGCAGCTAAATATCCCTTTT 45.0 38.1 57.8 55.2 970-1152 bp 1.0 52.9
ndhF-rpl32_p5 TGTTGCTGTCGGAATAAGCA TGGCAGCTAAATATCCCTTTTTC 45.0 39.1 57.8 57.1 970-1152 bp 1.0 52.3