Hotspot

trnL-CAA-ndhB

IRb · 97381-97957 · 577 bp

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Pi 0.00181

MarkerSeek score 63.20

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 577
variable_sites: 1
pi: 0.00181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TTCTTGCAATTGCTCAGGGT	50.0	45.0	58.0	58.0	618-641 bp	1.0	83.0
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TTCTTGCAATTGCTCAGGGT	50.0	45.0	58.0	58.0	616-639 bp	1.0	83.0
trnL-CAA-ndhB_p3	GCGTGTCTACCATTTCACCA	TTCTTGCAATTGCTCAGGGT	50.0	45.0	58.2	58.0	613-636 bp	1.0	81.5
trnL-CAA-ndhB_p4	TCTCGCGTGTCTACCATTTC	TTCTTGCAATTGCTCAGGGT	50.0	45.0	57.7	58.0	617-640 bp	1.0	80.9
trnL-CAA-ndhB_p5	TCGCGTGTCTACCATTTCAC	TTCTTGCAATTGCTCAGGGT	50.0	45.0	58.3	58.0	615-638 bp	1.0	80.7