Hotspot

petG-trnW-CCA

LSC · 69034-69149 · 116 bp

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Pi 0.069

MarkerSeek score 75.20

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 8
pi: 0.069
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	TTTACAATACAGACGCGGGG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	228 bp	1.0	82.6
petG-trnW-CCA_p2	TTTACAATACAGACGCGGGG	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.0	57.9	192 bp	1.0	82.5
petG-trnW-CCA_p3	GGGGATCAGTTGGACCTTTG	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.2	58.0	212 bp	1.0	81.6
petG-trnW-CCA_p4	GGGGATCAGTTGGACCTTTG	CAAAACCCGATGTCGTAGGT	55.0	50.0	58.2	57.9	176 bp	1.0	81.6
petG-trnW-CCA_p5	TTTACAATACAGACGCGGGG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	58.0	57.7	226 bp	1.0	79.9