Hotspot

psbF-psbE

LSC · 67416-67432 · 17 bp

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Pi 0.0588
MarkerSeek score 82.40
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
LSC
length_bp
17
variable_sites
1
pi
0.0588
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 ACGGTAGGTACAGCTAGTCC TAACCGGCCGTTTTGATTCT 55.0 45.0 58.0 57.8 147 bp 1.0 78.6
psbF-psbE_p2 GGTAGGTACAGCTAGTCCGT TAACCGGCCGTTTTGATTCT 55.0 45.0 58.0 57.8 145 bp 1.0 78.6
psbF-psbE_p3 ACGGTAGGTACAGCTAGTCC ATGTGTTTGGAAGTCCTCGG 55.0 50.0 58.0 57.8 210 bp 1.0 78.5
psbF-psbE_p4 GGTAGGTACAGCTAGTCCGT ATGTGTTTGGAAGTCCTCGG 55.0 50.0 58.0 57.8 208 bp 1.0 78.5
psbF-psbE_p5 GTCCGTGAACAGCTAACCAT ATGTGTTTGGAAGTCCTCGG 50.0 50.0 57.9 57.8 194 bp 1.0 78.2