primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	GGTATGGCTCCTTCAACGAT	GTGCTTGGGAGTCCTTGAAA	20	20	50.0	50.0	57.4	58.0	0.0	9.6	0.640807	97	1158	1154-1154	1.000000	74.9
psbA_p2	psbA	2	TGGCTCCTTCAACGATTCTT	GTGCTTGGGAGTCCTTGAAA	20	20	45.0	50.0	57.1	58.0	0.0	9.6	0.877909	97	1158	1150-1150	1.000000	73.0
psbA_p3	psbA	3	GGTATGGCTCCTTCAACGAT	TAGTACGTGTGCTTGGGAGT	20	20	50.0	50.0	57.4	58.4	0.0	0.0	0.997728	97	1158	1162-1162	1.000000	72.0
psbA_p4	psbA	4	GGTATGGCTCCTTCAACGAT	GTTAGTACGTGTGCTTGGGA	20	20	50.0	50.0	57.4	57.6	0.0	0.0	1.065951	97	1158	1164-1164	1.000000	71.5
psbA_p5	psbA	5	GGTATGGCTCCTTCAACGAT	AGTTAGTACGTGTGCTTGGG	20	20	50.0	50.0	57.4	57.5	0.0	0.0	1.072673	97	1158	1165-1165	1.000000	71.4
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGTCGCTTTAGTCCA	CGGACGAATCACACTTTTACC	20	21	50.0	47.6	58.0	57.3	0.0	0.0	1.721932	8348	9518	1192-1243	1.000000	71.3
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGTCGCTTTAGTCCAC	CGGACGAATCACACTTTTACC	20	21	50.0	47.6	58.0	57.3	0.0	0.0	1.723320	8348	9518	1191-1242	1.000000	71.3
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	AGCAATCCGTCGCTTTAGTC	CGGACGAATCACACTTTTACC	20	21	50.0	47.6	58.4	57.3	0.0	0.0	2.075773	8348	9518	1194-1245	1.000000	68.4
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	CGTCGCTTTAGTCCACTCAG	CGGACGAATCACACTTTTACC	20	21	55.0	47.6	58.4	57.3	0.0	0.0	2.098489	8348	9518	1187-1238	1.000000	68.3
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	GCTTTAGTCCACTCAGCCAT	CGGACGAATCACACTTTTACC	20	21	50.0	47.6	57.6	57.3	0.9	0.0	2.127298	8348	9518	1183-1234	1.000000	68.0
atpB-rbcL_p1	atpB-rbcL	1	GCCGACTGGAACACTTAGAG	CGGTCTCTCCAACGCATAAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.006913	56624	57871	2033-2207	1.000000	84.5
atpB-rbcL_p2	atpB-rbcL	2	GCCGACTGGAACACTTAGAG	AGATCCTCCAGACGTAGAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026671	56624	57871	1796-1970	1.000000	84.5
atpB-rbcL_p3	atpB-rbcL	3	GCCGACTGGAACACTTAGAG	ATCCTCCAGACGTAGAGCTC	20	20	55.0	55.0	58.0	58.0	0.0	5.3	0.026671	56624	57871	1794-1968	1.000000	84.5
atpB-rbcL_p4	atpB-rbcL	4	GCCGACTGGAACACTTAGAG	GATCCTCCAGACGTAGAGCT	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026671	56624	57871	1795-1969	1.000000	84.5
atpB-rbcL_p5	atpB-rbcL	5	GCCGACTGGAACACTTAGAG	GTCCACACAGTTGTCCATGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.035012	56624	57871	1595-1769	1.000000	84.5
psbF-psbE_p1	psbF-psbE	1	ACGGTAGGTACAGCTAGTCC	TAACCGGCCGTTTTGATTCT	20	20	55.0	45.0	58.0	57.8	0.0	26.1	0.237950	67416	67432	147-147	1.000000	78.6
psbF-psbE_p2	psbF-psbE	2	GGTAGGTACAGCTAGTCCGT	TAACCGGCCGTTTTGATTCT	20	20	55.0	45.0	58.0	57.8	0.0	26.1	0.237950	67416	67432	145-145	1.000000	78.6
psbF-psbE_p3	psbF-psbE	3	ACGGTAGGTACAGCTAGTCC	ATGTGTTTGGAAGTCCTCGG	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.234892	67416	67432	210-210	1.000000	78.5
psbF-psbE_p4	psbF-psbE	4	GGTAGGTACAGCTAGTCCGT	ATGTGTTTGGAAGTCCTCGG	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.234892	67416	67432	208-208	1.000000	78.5
psbF-psbE_p5	psbF-psbE	5	GTCCGTGAACAGCTAACCAT	ATGTGTTTGGAAGTCCTCGG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.279766	67416	67432	194-194	1.000000	78.2
petG-trnW-CCA_p1	petG-trnW-CCA	1	TTTACAATACAGACGCGGGG	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.0	58.0	12.5	2.6	0.028964	69034	69149	228-228	1.000000	82.6
petG-trnW-CCA_p2	petG-trnW-CCA	2	TTTACAATACAGACGCGGGG	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	58.0	57.9	12.5	1.4	0.101886	69034	69149	192-192	1.000000	82.5
petG-trnW-CCA_p3	petG-trnW-CCA	3	GGGGATCAGTTGGACCTTTG	AGCGCTCTTAGTTCAGTTCG	20	20	55.0	50.0	58.2	58.0	0.0	2.6	0.176604	69034	69149	212-212	1.000000	81.6
petG-trnW-CCA_p4	petG-trnW-CCA	4	GGGGATCAGTTGGACCTTTG	CAAAACCCGATGTCGTAGGT	20	20	55.0	50.0	58.2	57.9	0.0	1.4	0.249526	69034	69149	176-176	1.000000	81.6
petG-trnW-CCA_p5	petG-trnW-CCA	5	TTTACAATACAGACGCGGGG	CGCTCTTAGTTCAGTTCGGT	20	20	50.0	50.0	58.0	57.7	12.5	0.0	0.362234	69034	69149	226-226	1.000000	79.9
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.161494	69224	69391	234-235	1.000000	79.7
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.162838	69224	69391	235-236	1.000000	79.7
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.169530	69224	69391	223-224	1.000000	79.7
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.170864	69224	69391	221-222	1.000000	79.7
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.170864	69224	69391	224-225	1.000000	79.7
rpl22_p1	rpl22	1	CCAAGTCTGAAACCAAGTGGA	GATACTTACATTGGCGGGGG	21	20	47.6	55.0	58.1	58.1	3.8	0.0	1.176840	86305	86691	500-500	1.000000	71.5
rpl22_p2	rpl22	2	GTGCGAACCAAAAGGAATGA	GATACTTACATTGGCGGGGG	20	20	45.0	55.0	56.9	58.1	0.0	0.0	1.185535	86305	86691	535-535	1.000000	71.4
rpl22_p3	rpl22	3	CCAAGTCTGAAACCAAGTGGA	TTACATTGGCGGGGGATAAC	21	20	47.6	50.0	58.1	57.6	3.8	0.0	1.420756	86305	86691	495-495	1.000000	69.6
rpl22_p4	rpl22	4	GTGCGAACCAAAAGGAATGA	TTACATTGGCGGGGGATAAC	20	20	45.0	50.0	56.9	57.6	0.0	0.0	1.429450	86305	86691	530-530	1.000000	69.5
rpl22_p5	rpl22	5	ACCAAGTCTGAAACCAAGTGG	GATACTTACATTGGCGGGGG	21	20	47.6	55.0	58.3	58.1	0.0	0.0	1.452927	86305	86691	501-501	1.000000	69.3
trnH-GUG-rpl2_p1	trnH-GUG-rpl2	1	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.2	22.4	0.153095	87233	87279	197-197	1.000000	79.6
trnH-GUG-rpl2_p2	trnH-GUG-rpl2	2	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.157457	87233	87279	183-183	1.000000	79.6
trnH-GUG-rpl2_p3	trnH-GUG-rpl2	3	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	87233	87279	182-182	1.000000	79.6
trnH-GUG-rpl2_p4	trnH-GUG-rpl2	4	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	87233	87279	184-184	1.000000	79.6
trnH-GUG-rpl2_p5	trnH-GUG-rpl2	5	CGGATGTAGCCAAGTGGATC	TATCCTGCGCTTGGAAGAAG	20	20	55.0	50.0	58.1	57.7	0.2	22.4	0.444845	87233	87279	195-195	1.000000	77.3
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	TTCTTGCAATTGCTCAGGGT	20	20	50.0	45.0	58.0	58.0	5.8	29.4	0.008182	97381	97957	618-641	1.000000	83.0
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	TTCTTGCAATTGCTCAGGGT	20	20	50.0	45.0	58.0	58.0	5.8	29.4	0.009553	97381	97957	616-639	1.000000	83.0
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	GCGTGTCTACCATTTCACCA	TTCTTGCAATTGCTCAGGGT	20	20	50.0	45.0	58.2	58.0	0.0	29.4	0.197018	97381	97957	613-636	1.000000	81.5
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	TCTCGCGTGTCTACCATTTC	TTCTTGCAATTGCTCAGGGT	20	20	50.0	45.0	57.7	58.0	5.8	29.4	0.273721	97381	97957	617-640	1.000000	80.9
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	TCGCGTGTCTACCATTTCAC	TTCTTGCAATTGCTCAGGGT	20	20	50.0	45.0	58.3	58.0	5.8	29.4	0.295096	97381	97957	615-638	1.000000	80.7
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	111532	112135	745-745	1.000000	78.7
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	111532	112135	782-782	1.000000	78.1
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	111532	112135	696-696	1.000000	77.5
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	20	20	55.0	55.0	57.8	57.8	18.9	4.4	0.421214	111532	112135	697-697	1.000000	77.1
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.7	58.2	18.7	18.9	0.442458	111532	112135	733-733	1.000000	76.9
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCTGTCGGAATAAGCA	AGAAAAAGCTTTTATGGCAGC	20	21	45.0	38.1	57.8	55.6	7.1	24.1	3.532869	115696	116663	984-1166	1.000000	56.3
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCTGTCGGAATAAGCA	AAGAAAAAGCTTTTATGGCAGC	20	22	45.0	36.4	57.8	56.4	7.1	24.1	3.788194	115696	116663	985-1167	1.000000	54.2
ndhF-rpl32_p3	ndhF-rpl32	3	TGTTGCTGTCGGAATAAGCA	TGGCAGCTAAATATCCCTTT	20	20	45.0	40.0	57.8	54.4	7.1	0.0	3.825660	115696	116663	970-1152	1.000000	53.9
ndhF-rpl32_p4	ndhF-rpl32	4	TGTTGCTGTCGGAATAAGCA	TGGCAGCTAAATATCCCTTTT	20	21	45.0	38.1	57.8	55.2	7.1	0.0	3.959018	115696	116663	970-1152	1.000000	52.9
ndhF-rpl32_p5	ndhF-rpl32	5	TGTTGCTGTCGGAATAAGCA	TGGCAGCTAAATATCCCTTTTTC	20	23	45.0	39.1	57.8	57.1	7.1	0.0	4.029676	115696	116663	970-1152	1.000000	52.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGCAAAGTCAGTTTCTACCGG	TCCTAAGAGCAGCGTGTCTA	21	20	47.6	50.0	58.2	57.9	0.0	0.0	1.356773	116838	117631	841-906	1.000000	73.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCAAAGTCAGTTTCTACCGGA	TCCTAAGAGCAGCGTGTCTA	21	20	47.6	50.0	58.2	57.9	9.5	0.0	1.361532	116838	117631	840-905	1.000000	73.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCAAAGTCAGTTTCTACCGG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	56.7	57.9	0.0	0.0	1.377200	116838	117631	840-905	1.000000	73.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGCAAAGTCAGTTTCTACCGG	AGCAGCGTGTCTACCAATTT	21	20	47.6	45.0	58.2	57.8	0.0	0.0	1.417499	116838	117631	834-899	1.000000	73.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCAAAGTCAGTTTCTACCGGA	AGCAGCGTGTCTACCAATTT	21	20	47.6	45.0	58.2	57.8	9.5	0.0	1.422259	116838	117631	833-898	1.000000	73.2
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	AGCTATTGTTTCGTCGATCT	20	20	50.0	40.0	58.1	54.6	0.0	0.0	3.509435	120868	121542	740-850	1.000000	58.5
psaC-ndhE_p2	psaC-ndhE	2	CCAATACATCTGTGGGGCAA	AGCTATTGTTTCGTCGATCT	20	20	50.0	40.0	57.9	54.6	0.0	0.0	3.525921	120868	121542	717-827	1.000000	58.5
psaC-ndhE_p3	psaC-ndhE	3	AATACATCTGTGGGGCAAGC	AGCTATTGTTTCGTCGATCT	20	20	50.0	40.0	58.2	54.6	0.0	0.0	3.615806	120868	121542	715-825	1.000000	57.8
psaC-ndhE_p4	psaC-ndhE	4	AGCTTTACATCCGTCCCAAG	AGCTATTGTTTCGTCGATCT	20	20	50.0	40.0	57.6	54.6	0.0	0.0	3.786817	120868	121542	746-856	1.000000	56.3
psaC-ndhE_p5	psaC-ndhE	5	TTCCAATACATCTGTGGGGC	AGCTATTGTTTCGTCGATCT	20	20	50.0	40.0	57.6	54.6	0.0	0.0	3.818937	120868	121542	719-829	1.000000	56.1
rps15-ycf1_p1	rps15-ycf1	1	TCTTGTCGAATAGCTGAGATGA	ACCAATAACGGCGGTCATTT	22	20	40.9	45.0	56.7	58.2	0.0	13.2	3.426027	127380	127762	484-487	1.000000	55.7
rps15-ycf1_p2	rps15-ycf1	2	TCTTGTCGAATAGCTGAGATGA	GATACCAATAACGGCGGTCA	22	20	40.9	50.0	56.7	57.8	0.0	13.2	3.483144	127380	127762	487-490	1.000000	55.2
rps15-ycf1_p3	rps15-ycf1	3	TCTTGTCGAATAGCTGAGATGA	TGATACCAATAACGGCGGTC	22	20	40.9	50.0	56.7	57.8	0.0	13.2	3.483144	127380	127762	488-491	1.000000	55.2
rps15-ycf1_p4	rps15-ycf1	4	TCTTGTCGAATAGCTGAGATGA	ACGGCGGTCATTTCAGTATG	22	20	40.9	50.0	56.7	58.3	0.0	13.2	3.603019	127380	127762	477-480	1.000000	54.4
rps15-ycf1_p5	rps15-ycf1	5	TCTTGTCGAATAGCTGAGATGA	CGGCGGTCATTTCAGTATGT	22	20	40.9	50.0	56.7	58.3	0.0	0.0	3.603019	127380	127762	476-479	1.000000	54.4
ycf1_p1	ycf1	1	TCAACCAATGAAATACCTACGGT	ATGGAATGCCCCCTTGAATC	23	20	39.1	50.0	57.8	57.9	0.0	0.0	3.261245	128333	128452	261-261	1.000000	55.4
ycf1_p2	ycf1	2	TCAACCAATGAAATACCTACGGT	GATGGAATGCCCCCTTGAAT	23	20	39.1	50.0	57.8	57.9	0.0	0.0	3.261245	128333	128452	262-262	1.000000	55.4
ycf1_p3	ycf1	3	TCAACCAATGAAATACCTACGGT	GGAATGCCCCCTTGAATCAA	23	20	39.1	50.0	57.8	58.1	0.0	0.0	3.303978	128333	128452	259-259	1.000000	55.1
ycf1_p4	ycf1	4	CAACCAATGAAATACCTACGGT	ATGGAATGCCCCCTTGAATC	22	20	40.9	50.0	56.4	57.9	0.0	0.0	3.667716	128333	128452	260-260	1.000000	52.2
ycf1_p5	ycf1	5	CAACCAATGAAATACCTACGGT	GATGGAATGCCCCCTTGAAT	22	20	40.9	50.0	56.4	57.9	0.0	0.0	3.667716	128333	128452	261-261	1.000000	52.2
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	TTCTTGCAATTGCTCAGGGT	GTCTCGCGTGTCTACCATTT	20	20	45.0	50.0	58.0	58.0	29.4	5.8	0.008182	147754	148330	618-641	1.000000	83.0
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	TTCTTGCAATTGCTCAGGGT	CTCGCGTGTCTACCATTTCA	20	20	45.0	50.0	58.0	58.0	29.4	5.8	0.009553	147754	148330	616-639	1.000000	83.0
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	TTCTTGCAATTGCTCAGGGT	GCGTGTCTACCATTTCACCA	20	20	45.0	50.0	58.0	58.2	29.4	0.0	0.197018	147754	148330	613-636	1.000000	81.5
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	TTCTTGCAATTGCTCAGGGT	TCTCGCGTGTCTACCATTTC	20	20	45.0	50.0	58.0	57.7	29.4	5.8	0.273721	147754	148330	617-640	1.000000	80.9
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	TTCTTGCAATTGCTCAGGGT	TCGCGTGTCTACCATTTCAC	20	20	45.0	50.0	58.0	58.3	29.4	5.8	0.295096	147754	148330	615-638	1.000000	80.7
rpl2-trnH-GUG_p1	rpl2-trnH-GUG	1	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	22.4	0.2	0.153095	158432	158478	197-197	1.000000	79.6
rpl2-trnH-GUG_p2	rpl2-trnH-GUG	2	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.157457	158432	158478	183-183	1.000000	79.6
rpl2-trnH-GUG_p3	rpl2-trnH-GUG	3	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	158432	158478	182-182	1.000000	79.6
rpl2-trnH-GUG_p4	rpl2-trnH-GUG	4	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	158432	158478	184-184	1.000000	79.6
rpl2-trnH-GUG_p5	rpl2-trnH-GUG	5	TATCCTGCGCTTGGAAGAAG	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	57.7	58.1	22.4	0.2	0.444845	158432	158478	195-195	1.000000	77.3