Hotspot

rps15-ycf1

SSC · 122045-122430 · 386 bp

Back to Bonnaya

Pi 0.0259

MarkerSeek score 65.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 386
variable_sites: 15
pi: 0.0259
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGACCCTCTGTTTTCTTCGT	GATCCGTTTGTACCCACGAT	47.6	50.0	57.9	57.7	487-489 bp	1.0	71.1
rps15-ycf1_p2	CAGACCCTCTGTTTTCTTCGT	AAGGATCCGTTTGTACCCAC	47.6	50.0	57.9	57.5	490-492 bp	1.0	69.7
rps15-ycf1_p3	CAGACCCTCTGTTTTCTTCGT	ATCCGTTTGTACCCACGATT	47.6	45.0	57.9	57.2	486-488 bp	1.0	67.4
rps15-ycf1_p4	AGACCCTCTGTTTTCTTCGT	GATCCGTTTGTACCCACGAT	45.0	50.0	56.4	57.7	486-488 bp	1.0	67.3
rps15-ycf1_p5	AGACCCTCTGTTTTCTTCGT	AAGGATCCGTTTGTACCCAC	45.0	50.0	56.4	57.5	489-491 bp	1.0	65.8