Hotspot

ndhG-ndhI

SSC · 117388-117735 · 348 bp

Back to Bonnaya

Pi 0.0175
MarkerSeek score 68.50
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
348
variable_sites
9
pi
0.0175
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AAGACCCGATCCCAGAAAGA TCAAATTGCTTTAGGTCGGT 50.0 40.0 58.0 55.3 487-499 bp 1.0 61.3
ndhG-ndhI_p2 AAGACCCGATCCCAGAAAGA TCAAATTGCTTTAGGTCGGTT 50.0 38.1 58.0 56.1 487-499 bp 1.0 59.8
ndhG-ndhI_p3 TCCCAATGAAAAGGCCGAAT TCAAATTGCTTTAGGTCGGT 45.0 40.0 57.8 55.3 550-562 bp 1.0 59.4
ndhG-ndhI_p4 AAGACCCGATCCCAGAAAGA ATCAAATTGCTTTAGGTCGGT 50.0 38.1 58.0 55.8 488-500 bp 1.0 58.0
ndhG-ndhI_p5 TCCCAATGAAAAGGCCGAAT TCAAATTGCTTTAGGTCGGTT 45.0 38.1 57.8 56.1 550-562 bp 1.0 57.9