Hotspot

rpl32-trnL-UAG

SSC · 112061-112852 · 792 bp

Back to Bonnaya

Pi 0.0159

MarkerSeek score 67.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 792
variable_sites: 15
pi: 0.0159
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CCCTTTCAACCGGGAATTCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	509-889 bp	1.0	84.9
rpl32-trnL-UAG_p2	CCCTTTCAACCGGGAATTCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	510-890 bp	1.0	84.8
rpl32-trnL-UAG_p3	CCCTTTCAACCGGGAATTCA	TTTAGGAGATGACATGCCGC	50.0	50.0	58.1	58.0	574-954 bp	1.0	84.4
rpl32-trnL-UAG_p4	CCCTTTCAACCGGGAATTCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	519-899 bp	1.0	83.9
rpl32-trnL-UAG_p5	TCCCTTTCAACCGGGAATTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	510-890 bp	1.0	83.8