Hotspot

psbT-psbN

LSC · 74778-74843 · 66 bp

Back to Bonnaya

Pi 0.0505

MarkerSeek score 68.10

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 5
pi: 0.0505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GGGTACGCCTTATATACCGC	50.0	55.0	57.6	57.7	175 bp	1.0	77.1
psbT-psbN_p2	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	50.0	55.0	57.6	58.4	127 bp	1.0	76.9
psbT-psbN_p3	TCGAGAACCGCCTAAAGTTC	GTTTTACCGGGTACGCCTTA	50.0	50.0	57.6	57.6	183 bp	1.0	76.3
psbT-psbN_p4	TCGAGAACCGCCTAAAGTTC	TAAGTTTTACCGGGTACGCC	50.0	50.0	57.6	57.6	186 bp	1.0	76.3
psbT-psbN_p5	GAGAACCGCCTAAAGTTCCA	GGGTACGCCTTATATACCGC	50.0	55.0	57.5	57.7	173 bp	1.0	76.3