Hotspot

petG-trnW-CCA

LSC · 66721-66808 · 88 bp

Back to Bonnaya

Pi 0.0303

MarkerSeek score 66.60

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 88
variable_sites: 4
pi: 0.0303
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CAATACAGGCGTGGTGATCA	GGTTCAAGTCCTACAGAGCG	50.0	55.0	58.0	58.0	143-145 bp	1.0	82.0
petG-trnW-CCA_p2	ACAATACAGGCGTGGTGATC	GGTTCAAGTCCTACAGAGCG	50.0	55.0	58.0	58.0	144-146 bp	1.0	82.0
petG-trnW-CCA_p3	CAATACAGGCGTGGTGATCA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	174-176 bp	1.0	81.4
petG-trnW-CCA_p4	CAATACAGGCGTGGTGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	196-198 bp	1.0	81.3
petG-trnW-CCA_p5	ACAATACAGGCGTGGTGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	197-199 bp	1.0	81.3