Hotspot

trnG-CGA-trnR-UCU

LSC · 10015-10242 · 228 bp

Back to Bonnaya

Pi 0.077

MarkerSeek score 79.20

Primer available yes

Label trnG-CGA-trnR-UCU

Feature Metadata

label_name: trnG-CGA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 228
variable_sites: 24
pi: 0.077
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-CGA-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	280-308 bp	1.0	73.9
trnG-CGA-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	286-314 bp	1.0	73.1
trnG-CGA-trnR-UCU_p3	CTAACGATGCGGGTTCGATT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.4	56.9	269-297 bp	1.0	70.9
trnG-CGA-trnR-UCU_p4	TAACGATGCGGGTTCGATTC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.4	56.9	268-296 bp	1.0	70.9
trnG-CGA-trnR-UCU_p5	CTAACGATGCGGGTTCGATT	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.4	57.9	275-303 bp	1.0	70.1