Hotspot

rps16-trnQ-UUG

LSC · 6125-7260 · 1136 bp

Back to Bonnaya

Pi 0.022
MarkerSeek score 60.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1136
variable_sites
37
pi
0.022
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAAATCAAAATGGGGCGTGG 50.0 50.0 57.9 58.0 1274-1285 bp 1.0 82.9
rps16-trnQ-UUG_p2 AGTCCCGGTTATTGAGACCA GAAATCAAAATGGGGCGTGG 50.0 50.0 58.1 58.0 1777-1788 bp 1.0 82.7
rps16-trnQ-UUG_p3 AGTCCCGGTTATTGAGACCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 1719-1730 bp 1.0 82.3
rps16-trnQ-UUG_p4 AGTCCCGGTTATTGAGACCA AGCAGCTTGCCAAACAAAAG 50.0 45.0 58.1 58.1 2278-2288 bp 1.0 82.1
rps16-trnQ-UUG_p5 AGTCCCGGTTATTGAGACCA CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.1 57.9 2283-2293 bp 1.0 82.1