Hotspot

trnH-GUG-psbA

LSC · 86-634 · 549 bp

Back to Bonnaya

Pi 0.0426

MarkerSeek score 78.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 549
variable_sites: 34
pi: 0.0426
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	664-669 bp	1.0	83.5
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	55.0	50.0	58.1	58.2	666-671 bp	1.0	83.4
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	678-683 bp	1.0	83.3
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	677-682 bp	1.0	83.3
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	679-684 bp	1.0	83.3