Hotspot

rps18

LSC · 67325-67858 · 534 bp

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Pi 0
MarkerSeek score 61.20
Primer available yes
Label rps18

Feature Metadata

label_name
rps18
feature_type
gene
region
LSC
length_bp
534
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18_p1 AGACTACTTCTTCTGGATCCA GGTCTGGCCTTTTCGAATAA 42.9 45.0 55.1 55.7 615-635 bp 1.0 42.7
rps18_p2 AAGACTACTTCTTCTGGATCCA GGTCTGGCCTTTTCGAATAA 40.9 45.0 55.9 55.7 616-636 bp 1.0 42.7
rps18_p3 AGACTACTTCTTCTGGATCCAA GGTCTGGCCTTTTCGAATAA 40.9 45.0 55.9 55.7 615-635 bp 1.0 42.7
rps18_p4 AGACTACTTCTTCTGGATCCA GGTCTGGCCTTTTCGAATAAA 42.9 42.9 55.1 56.5 615-635 bp 1.0 42.7
rps18_p5 TGGACCAAAGAAAGACTACTTCT GGTCTGGCCTTTTCGAATAA 39.1 45.0 57.1 55.7 627-647 bp 1.0 42.6