Hotspot

trnL-CAA-trnI-CAU

IRa · 133165-135670 · 2506 bp

Back to Bonia

Pi 0.00161
MarkerSeek score 58.30
Primer available yes
Label trnL-CAA-trnI-CAU

Feature Metadata

label_name
trnL-CAA-trnI-CAU
feature_type
igs
region
IRa
length_bp
2506
variable_sites
4
pi
0.00161
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-trnI-CAU_p1 AAATGGTAGACACGCGAGAC GTTGGGCGCTTTAACCATTC 50.0 50.0 58.0 58.0 2588-2607 bp 1.0 80.6
trnL-CAA-trnI-CAU_p2 TGAAATGGTAGACACGCGAG GTTGGGCGCTTTAACCATTC 50.0 50.0 58.0 58.0 2590-2609 bp 1.0 80.6
trnL-CAA-trnI-CAU_p3 TGGTGAAATGGTAGACACGC GTTGGGCGCTTTAACCATTC 50.0 50.0 58.2 58.0 2593-2612 bp 1.0 79.1
trnL-CAA-trnI-CAU_p4 GAAATGGTAGACACGCGAGA GTTGGGCGCTTTAACCATTC 50.0 50.0 57.7 58.0 2589-2608 bp 1.0 78.4
trnL-CAA-trnI-CAU_p5 GTTCGAGTCCTCTTCAAGGC GTTGGGCGCTTTAACCATTC 55.0 50.0 58.3 58.0 2540-2559 bp 1.0 78.4