Hotspot

petA-psbJ

LSC · 62143-63125 · 983 bp

Back to Bonia

Pi 0.0061
MarkerSeek score 62.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
983
variable_sites
6
pi
0.0061
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGCATCCGTTATTTTGGCAC TCCTCTTTGGCTGATAGGTACT 50.0 45.5 58.5 58.0 1168 bp 1.0 60.7
petA-psbJ_p2 CGCATCCGTTATTTTGGCAC CCTCTTTGGCTGATAGGTACTG 50.0 50.0 58.5 57.9 1167 bp 1.0 60.2
petA-psbJ_p3 TCGCATCCGTTATTTTGGCA TCCTCTTTGGCTGATAGGTACT 45.0 45.5 58.5 58.0 1169 bp 1.0 60.0
petA-psbJ_p4 TTCGCATCCGTTATTTTGGC TCCTCTTTGGCTGATAGGTACT 45.0 45.5 57.4 58.0 1170 bp 1.0 60.0
petA-psbJ_p5 GCGTCCAAGGCCTTTTATTC TCCTCTTTGGCTGATAGGTACT 50.0 45.5 57.4 58.0 1193 bp 1.0 59.9