Hotspot

clpP1

LSC · 74931-77096 · 2166 bp

Back to Bombycidendron

Pi 0.0107
MarkerSeek score 49.80
Primer available yes
Label clpP1

Feature Metadata

label_name
clpP1
feature_type
gene
region
LSC
length_bp
2166
variable_sites
23
pi
0.0107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP1_p1 GTGAACGGAAGAACCCCATT ATACGCAATGGGGGTTGATC 50.0 50.0 58.1 58.0 2827-2829 bp 1.0 81.2
clpP1_p2 GTGAACGGAAGAACCCCATT ATACCTCCCCTTTTCGGTGA 50.0 50.0 58.1 58.0 2956-2958 bp 1.0 81.1
clpP1_p3 GTGAACGGAAGAACCCCATT TACCTCCCCTTTTCGGTGAT 50.0 50.0 58.1 58.0 2955-2957 bp 1.0 81.1
clpP1_p4 CTAGTACATGTTCCTCGGCG ATACGCAATGGGGGTTGATC 55.0 50.0 57.9 58.0 2724-2726 bp 1.0 81.1
clpP1_p5 CTAGTACATGTTCCTCGGCG TACCTCCCCTTTTCGGTGAT 55.0 50.0 57.9 58.0 2852-2854 bp 1.0 80.9