Hotspot

ccsA-ndhD

SSC · 122296-122609 · 314 bp

Back to Bombycidendron

Pi 0.0353

MarkerSeek score 63.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 314
variable_sites: 11
pi: 0.0353
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GTAAATTCCGCACTTGTGGC	TCAGGTGAGAAGGTCGAAAC	50.0	50.0	58.0	57.2	482-490 bp	1.0	75.4
ccsA-ndhD_p2	GTAAATTCCGCACTTGTGGC	CAGGTGAGAAGGTCGAAACT	50.0	50.0	58.0	57.2	481-489 bp	1.0	75.4
ccsA-ndhD_p3	TAAATTCCGCACTTGTGGCT	TCAGGTGAGAAGGTCGAAAC	45.0	50.0	58.1	57.2	481-489 bp	1.0	74.9
ccsA-ndhD_p4	TAAATTCCGCACTTGTGGCT	CAGGTGAGAAGGTCGAAACT	45.0	50.0	58.1	57.2	480-488 bp	1.0	74.8
ccsA-ndhD_p5	GTAAATTCCGCACTTGTGGC	ATCAGGTGAGAAGGTCGAAAC	50.0	47.6	58.0	57.7	483-491 bp	1.0	71.4