Hotspot

rps12-clpP1

LSC · 74744-74930 · 187 bp

Back to Bombycidendron

Pi 0.0267

MarkerSeek score 66.40

Primer available yes

Label rps12-clpP1

Feature Metadata

label_name: rps12-clpP1
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 5
pi: 0.0267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP1_p1	CTAGTACATGTTCCTCGGCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.9	57.9	352 bp	1.0	79.3
rps12-clpP1_p2	CCTAGTACATGTTCCTCGGC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.5	57.9	353 bp	1.0	76.1
rps12-clpP1_p3	GCAGGGGATTTCGTGACATT	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.5	57.9	313 bp	1.0	76.1
rps12-clpP1_p4	CTAGTACATGTTCCTCGGCG	TTGTGGATCATGTAGCGGTT	55.0	45.0	57.9	57.2	320 bp	1.0	74.0
rps12-clpP1_p5	CTAGTACATGTTCCTCGGCG	GCCCAAGCTCATGGAATTGT	55.0	50.0	57.9	58.8	336 bp	1.0	73.7