Hotspot

rps18-rpl20

LSC · 73226-73521 · 296 bp

Back to Bombycidendron

Pi 0.0274

MarkerSeek score 64.90

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 296
variable_sites: 8
pi: 0.0274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	ACCGCTAGAACTACTGCTCT	AACAGTTGCTTCTGAATCGT	50.0	40.0	57.9	55.6	427-429 bp	1.0	62.8
rps18-rpl20_p2	ACCGCTAGAACTACTGCTCT	GAAACAGTTGCTTCTGAATCGT	50.0	40.9	57.9	57.5	429-431 bp	1.0	62.1
rps18-rpl20_p3	TTGAAAGAAGCGAGTCGACC	AACAGTTGCTTCTGAATCGT	50.0	40.0	58.2	55.6	444-446 bp	1.0	61.9
rps18-rpl20_p4	TTGAAAGAAGCGAGTCGACC	GAAACAGTTGCTTCTGAATCGT	50.0	40.9	58.2	57.5	446-448 bp	1.0	61.3
rps18-rpl20_p5	ACCGCTAGAACTACTGCTCT	AAACAGTTGCTTCTGAATCGT	50.0	38.1	57.9	56.4	428-430 bp	1.0	61.0