Hotspot

rpl33-rps18

LSC · 72447-72919 · 473 bp

Back to Bombycidendron

Pi 0.0402

MarkerSeek score 80.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 16
pi: 0.0402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TACGCCTAGTCGATTGGAGT	TCAATTCGATCCCCCGATTG	50.0	50.0	58.0	57.7	596-638 bp	1.0	83.7
rpl33-rps18_p2	TACGCCTAGTCGATTGGAGT	ATTCGATCCCCCGATTGGAT	50.0	50.0	58.0	58.6	593-635 bp	1.0	80.6
rpl33-rps18_p3	CGCCTAGTCGATTGGAGTTG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.4	57.7	594-636 bp	1.0	80.5
rpl33-rps18_p4	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.5	57.7	604-646 bp	1.0	79.7
rpl33-rps18_p5	TACGCCTAGTCGATTGGAGT	CAATTCGATCCCCCGATTGG	50.0	55.0	58.0	58.8	595-637 bp	1.0	79.6